In most labs, yes. XUP™ is a petroleum-based hydrocarbon clearing reagent that is fully miscible with xylene, so the switch starts from the protocol you already run.
Start with your current times. Run XUP™ at the same clearing times as your xylene protocol. For biopsy programs, the clearing step can typically be reduced to about 30 minutes once results are confirmed.
No processor flush required. Because XUP™ mixes fully with xylene, residual xylene left in the processor does not cause problems during changeover.
Tested processors. Compatibility testing is complete on the Leica HistoCore PELORIS, Sakura Tissue-Tek VIP and Dakewe HP300. On processors with reagent density sensors, such as the PELORIS, change the reagent setting from xylene to another solvent type so level readings stay correct.
One known limitation. On film coverslippers and on systems such as the Leica Spectra, keep the final slide-holding chamber filled with xylene, because XUP™ does not dissolve film resin quickly enough. On the Tissue-Tek Glas G2 glass coverslipper, XUP™ and XUP™ Mount run without that change.
Recycling and safety. XUP™ works with standard solvent recyclers used for xylene. It is still a flammable liquid (GHS Category 3, UN 1268), so storage and disposal follow the SDS and local rules.
Verify on your own tissue. Any reagent change needs verification against your lab’s acceptance criteria. Our Stain Evaluation Support matches your current staining on your own blocks before you switch. Request an XUP™ evaluation sample.
